UBE2F Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD83181A
Immunofluorescence analysis of NIH-3T3 cells using UBE2F Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
UBE2F Polyclonal Antibody
Catalog Number
RD83181A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Immunogen Information
Immunogen
Recombinant fusion protein of human UBE2F (NP_542409.1).
Gene ID
140739
Swissprot
Q969M7
Synonyms
UBE2FNCE2
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IF
Conjugation
Unconjugated
Dilution
IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas