UBE2I Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD82594A
Western blot analysis of extracts of various cell lines using UBE2I Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using UBE2I Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat brain using UBE2I Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using UBE2I Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using UBE2I Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using UBE2I Polyclonal Antibody at dilution of 1:100 (40x lens).
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Overview

Circle
Product Name
UBE2I Polyclonal Antibody
Catalog Number
RD82594A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human UBE2I (NP_003336.1).
Gene ID
7329
Swissprot
P63279
Synonyms
UBE2IC358B7.1P18UBC9
Calculated MW
18 kDa
Observed MW
18 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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