UCHL1 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD90661A
Western blot with UCHL1 Polyclonal antibody at dilution of 1:1000.lane 1:SH-SY5Y whole cell lysate, lane 2:Rat brain, lane 3:C6 whole cell lysate
Western blot with UCHL1 Polyclonal antibody at dilution of 1:1000.lane 1:SH-SY5Y whole cell lysate, lane 2:Rat brain, lane 3:C6 whole cell lysate
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Overview

Circle
Product Name
UCHL1 Polyclonal Antibody
Catalog Number
RD90661A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.Also binds to free monoubiquitin and may prevent its degradation in lysosomes.The homodimer may have ATP-independent ubiquitin ligase activity

Immunogen Information

Circle
Immunogen
Recombinant Human UCHL1 protein expressed by E.coli
Swissprot
P09936
Synonyms
UCHL1UCH-L1EC:3.4.19.12Ubiquitin carboxyl-terminal hydrolase isozyme L1Neuron cytoplasmic protein 9.5PGP 9.5 (PGP9.5)Ubiquitin thioesterase L1
Calculated MW
11 kDa
Observed MW
14 kDa

Applications

Circle
Reactivity
Human,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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