USO1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD266683A
Western blot analysis of extracts of various cell lines using USO1 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of HeLa cells using USO1 Polyclonal Antibody at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using USO1 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using USO1 Polyclonal Antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Product Name
USO1 Polyclonal Antibody
Catalog Number
RD266683A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human USO1 (NP_003706.2).
Gene ID
8615
Swissprot
O60763
Synonyms
USO1P115TAPVDP
Calculated MW
107 kDa/109 kDa
Observed MW
108 kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas