USP14 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD218519A
Western blot analysis of A172 and Jurkat cell lysates using USP14 Polyclonal Antibody at dilution of 1:400
Product Name
USP14 Polyclonal Antibody
Catalog Number
RD218519A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Immunogen Information
Immunogen
Fusion protein of human USP14
Swissprot
P54578
Synonyms
Deubiquitinating enzyme 14TGTtRNA guanine transglycosylase 60 kD subunittRNA guanine transglycosylaseUbiquitin carboxyl terminal hydrolase 14Ubiquitin carboxyl-terminal hydrolase 14Ubiquitin specific peptidase 14Ubiquitin specific processing protea
Calculated MW
56 kDa
Observed MW
Refer to figures
Gene Accession
BC003556
Applications
Reactivity
Human, Mouse
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, ELISA 1:5000-1:10000
Concentration
0.7 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas