For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88207A
Western blot analysis of extracts of various cell lines using USP47 Polyclonal Antibody at1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human colon carcinoma using USP47 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB, stabilizing POLB thereby playing a role in base-excision repair (BER. Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.
Immunogen Information
Immunogen
Recombinant fusion protein of human USP47
Gene ID
55031
Swissprot
Q96K76
Synonyms
USP47TRFP
Calculated MW
19kDa/20kDa/24kDa/25kDa/34kDa
Observed MW
15kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:200-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.