V5-Tag Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86706A
Western blotting with anti-V5-Tag monoclonal antibody at dilution of 1:1000.Lane 1: V5 tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
Product Name
V5-Tag Monoclonal Antibody
Catalog Number
RD86706A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG1
Host
Mouse
Background
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD)
Immunogen Information
Immunogen
Synthetic peptide corresponding to V5 tag conjugated to keyhole limpet haemocyanin.
Synonyms
GKPIPNPLLGLDST epitope tagGKPIPNPLLGLDST tagProtein RevRegulator of expression of viral proteinsrevV5 epitope tag
Calculated MW
26kDa
Observed MW
26kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IP 3ug/sample, IF 1:100-1:200
Concentration
4mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.