V5-Tag Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blotting of V5-Tag fused recombinant protein with Anti-V5 polyclonal antibody at dilution of
lane 1: 1:4000, lane 2:1:2000, lane 3 1:1000.
Product Name
V5-Tag Polyclonal Antibody
Catalog Number
RD86616A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9
Immunogen Information
Immunogen
Synthetic peptide corresponding to V5 tag conjugated to keyhole limpet haemocyanin.
Synonyms
GKPIPNPLLGLDST epitope tagGKPIPNPLLGLDST tagProtein RevRegulator of expression of viral proteinsrevV5 epitope tag
Calculated MW
30kDa
Observed MW
30kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:2000-1:5000
Concentration
2mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.