VDR Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using VDR Polyclonal Antibody at 1:500 dilution.
Western blot analysis of extracts of various cell lines using VDR Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded Human liver cancer using VDR Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human liver cancer using VDR Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse liver using VDR Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse liver using VDR Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat liver using VDR Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat liver using VDR Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of PC-12 using VDR Polyclonal Antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 using VDR Polyclonal Antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 using VDR Polyclonal Antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 using VDR Polyclonal Antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa using VDR Polyclonal Antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa using VDR Polyclonal Antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
VDR Polyclonal Antibody
Catalog Number
RD90076A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human VDR
Gene ID
7421
Swissprot
P11473
Synonyms
VDRNR1I1PPP1R163
Calculated MW
48kDa/53kDa
Observed MW
48KDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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