VPS25 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD81243A
Western blot analysis of extracts of various cell lines using VPS25 Polyclonal Antibody at dilution of 1:3000.
Immunofluorescence analysis of C6 cells using VPS25 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using VPS25 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
VPS25 Polyclonal Antibody
Catalog Number
RD81243A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1.
Immunogen Information
Immunogen
Recombinant fusion protein of human VPS25 (NP_115729.1).
Gene ID
84313
Swissprot
Q9BRG1
Synonyms
VPS25DERP9EAP20FAP20
Calculated MW
20 kDa
Observed MW
20 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas