VRK1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD252422A
Western blot analysis of 293T and 231 cell lysates using VRK1 Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using VRK1 Polyclonal Antibody at dilution of 1:30(×200)
Product Name
VRK1 Polyclonal Antibody
Catalog Number
RD252422A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
Immunogen Information
Immunogen
Fusion protein of human VRK1
Swissprot
Q99986
Synonyms
MGC117401MGC138280MGC142070PCH1PCH1ASerine/threonine protein kinase VRK1Serine/threonine-protein kinase VRK1Vaccinia related kinase 1Vaccinia virus B1R related kinase 1Vaccinia-related kinase 1VRK1VRK1
Calculated MW
45 kDa
Observed MW
Refer to figures
Gene Accession
BC113510
Applications
Reactivity
Human, Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000
Concentration
0.5 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.