WTAP Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90131A
Western blot analysis of extracts of various cell lines using WTAP Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of HeLa cells using WTAP Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using WTAP Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
WTAP Polyclonal Antibody
Catalog Number
RD90131A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.
Immunogen Information
Immunogen
Recombinant fusion protein of human WTAP
Gene ID
9589
Swissprot
Q15007
Synonyms
WTAPMum2
Calculated MW
154kDa
Observed MW
110kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas