Western blot analysis of extracts of various cell lines using XBP1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of 293T cells using XBP1 Polyclonal Antibody at 1:1000 dilution.293T cells were treated by tunicamycin (2 μg/ml) for 8 hours.
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
Immunogen Information
Immunogen
Recombinant fusion protein of human XBP1
Gene ID
7494
Swissprot
P17861
Synonyms
XBP1TREB-5TREB5XBP-1XBP2XBP1sCBX1
Calculated MW
49kDa/58kDa/199kDa
Observed MW
75KDa/280KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.