YWHAZ Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using YWHAZ Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using YWHAZ Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat brain using YWHAZ Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using YWHAZ Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using YWHAZ Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using YWHAZ Polyclonal Antibody at dilution of 1:100 (40x lens).
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Overview

Circle
Product Name
YWHAZ Polyclonal Antibody
Catalog Number
RD84604A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Immunogen Information

Circle
Immunogen
A synthetic peptide of human YWHAZ (NP_001129171.1).
Gene ID
7534
Swissprot
P63104
Synonyms
YWHAZ14-3-3-zetaHEL-S-3HEL-S-93HEL4KCIP-1YWHAD
Calculated MW
19 kDa/27 kDa
Observed MW
28 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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