Western blot analysis of extracts of various cell lines using ZNF263 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human colon using ZNF263 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Transcription factor that binds to the consensus sequence 5'-TCCTCCC-3' and acts as a transcriptional repressor. Binds to the promoter region of SIX3 and recruits other proteins involved in chromatin modification and transcriptional corepression, resulting in methylation of the promoter and transcriptional repression. Acts as transcriptional repressor of HS3ST1 and HS3ST3A1 via binding to gene promoter regions.
Immunogen Information
Immunogen
Recombinant fusion protein of human ZNF263
Gene ID
10127
Swissprot
O14978
Synonyms
ZNF263FPM315ZKSCAN12ZSCAN44
Calculated MW
120kDa
Observed MW
140kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.